Composite

Part:BBa_K1989002:Design

Designed by: Shijun Zhao   Group: iGEM16_Peking   (2016-10-12)


Triplespytag with mSA and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 486
    Illegal AgeI site found at 546
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of the linker between spycatcher may affect the physical and chemical property of hydrogel.



Source

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant

References